The present study explored the protective effects and the possible mechanisms of Sal on concanavalin A (ConA)‑induced liver injury in mice. Therefore, the model might allow the study of the pathophysiology of immunologically mediated hepatic disorders, such as AIH. Concanavalin A (Con-A) (1 μg/ml final, Amersham Biosciences, Uppsala, Sweden) was used as a positive control and background wells were added with CM only. Lithium interferes with transmembrane sodium exchange in nerve cells by affecting sodium, potassium-stimulated adenosine triphosphatase (Na+, K+-ATPase); alters the release of neurotransmitters; affects cyclic adenosine monophosphate concentrations; and … Dwyer JM. Protein threading is based on the basic observation that the number of different folds in nature is small (approximately 1300). 245(1):170-8. Trends Neurosci. Two of these, a site adjacent to the carbohydrate-specific site that interacts with phenyl β-glycosides of mannose, glucose and N-acetylglucosamine [84], and 4 low affinity sites (one per subunit) that bind various non-polar molecules such as tryptophan and indoleacetic acid [85], are of special interest. Thus, although ConA can trigger polyclonal T cell activation irrespective of their specificity, tumor specific T cells can be expanded and activated by ConA, whereby their antigen specificity is retained (Fig. Lectin of Concanavalin A as an anti-hepatoma therapeutic agent. Found inside – Page 11Since concanavalin A is present as a tetramer and every unit has a binding site for glucose, concanavalin A can function as a cross-linking agent for ... The automated HPLC platform integrated with inline MLAC enrichment was shown to generate high recovery, high throughput for clinical studies and disease related biomarker analysis (Kullolli, Hancock, & Hincapie, 2010; Zeng et al., 2011). It is a measure of the deviation between the position of an atom and some reference position (a measure of the average atomic mobility). Accessibility Dissolve 50 μg sample in loading buffer (injection volume depends on the volume held by the sample loop). This method was optimized to a high-performance multi-lectin affinity chromatography (HP-MLAC) by incorporating commercial ConA, WGA and JAC to a HPLC column (Kullolli, Hancock, & Hincapie, 2008). Kainate receptors are members of the ionotropic glutamate receptor family. concanavalin A: ( kon'kă-nav'ă-lin ), A phytomitogen, extracted from the jack bean ( Canavalia ensiformis ) that agglutinates the blood of mammals and reacts with glucosans; like other phytohemagglutinins, conA stimulates T lymphocytes more vigorously than it does B lymphocytes. The degree of connectivity of this postulated network appears to be altered by changes in the state of attachment of various surface receptors to the network. The enhancement of the suppressive effects of PRG 25-C was not observed when murine T cells were activated with phytohemagglutinin, anti-CD3 antibody, or allogeneic antigen presenting cells [40]. Found insideThis book will be of interest to practitioners in the fields of biology and medicine. The concanavalin A model of acute hepatitis in mice F Heymann1, K Hamesch1, R Weiskirchen2 and F Tacke1 Abstract The intravenous injection of the plant lectin concanavalin A (ConA) is a widely used model for acute immune-mediated hepatitis in mice. Metal Ion Binding and Conformational Transitions in Concanavalin A: A Structure-Function Study. Glycophorin (which is the M/N blood group determinant) and the concanavalin A receptor were isolated and purified prior to incorporation into model membranes by dialytic removal of detergent from lipid/protein solutions. Finding an effective way to manage ALF is important. However, the coupling reactions are sufficiently reproducible and rapid that we find no advantage to prior preparation of ConA-modified surfaces. Found inside – Page 117Fig.6.17 shows the binding of erythrocytes and thymocytes on various fibres as a function of the number of concanavalin A molecules bound per centimetre of ... The proteins with prosurvival ( green) or prodeath ( red) roles act as causative or contributing role on ConA treatment, including apoptosis, autophagic cell death, and antiangiogenesis. Tracking of Glycans Structure and Metallomics Profiles in. In addition to its mitogenic activity, ConA can induce programmed cell death via mitochondria-mediated apoptosis and autophagy [2-4]. The oligosaccharides maltose, maltotriose, mannotriose, and mannotetrose have been chemically attached to insulin molecules. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.PI binds to DNA by intercalating between the bases with little or no sequence preference. (B) ConA stimulates the expansion of cancer-specific CTLs in vitro. Indeed, in both studies ConA did induce minor off-target cytotoxicity caused by polyclonal expansion, but only when the ConA incubation time and effector-to-target ratio were strongly increased [75, 76]. The latter is in line with the reported autophagic cell death in cancer cells, which also depended on ConA internalization (see above section). The Surfactant-Induced Conformational and Activity Alterations in Rhizopus niveus Lipase. Pretreatment with dihydroquercetin, a dietary flavonoid, protected against concanavalin A-induced immunological hepatic injury in mice and TNF-α/ActD-induced apoptosis in HepG2 cells Jiajie Chen , a Xu Sun , a Tingting Xia , a Qiqi Mao a and Liang Zhong * a TNF-α and IFN-γ play important roles in Con A–induced liver injury (Gantner et al., 1995; Kusters et al., 1996) and IL-10 prevents liver injury in this model (Di Marco et al., 1999; Louis et al., 1997). α-Mannosyl residues substituted at their C-2 position also bind to Con A [79]. stimulation of mouse T cells using Concanavalin A in vitro. Therefore, the binding selectivity of lectins to glycopeptides is highly diverse and depends on the structures of both lectin and the glycans. This site needs JavaScript to work properly. Would you like email updates of new search results? SLAC offers more flexibility in the design of enrichment workflow with some specific goals. Concanavalin A (Con A) _______ is a plant lectin protein found in jack-bean that binds to mannose and glucose residues of glycoproteins. Enhanced Concanavalin A Binding to Preorganized Mannose Nanoarrays in Glycodendrimersomes Revealed Multivalent Interactions. This response was antigen-specific, as there was no toxicity toward another lymphoma cell line. Karl D. Hardman. Prepare methyl-BSA-IFA solution same as CFA solution above. First, the surface is activated by injection of a mixture of 0.2 M EDC with 50 mM NHS in water at a flow rate of 5 μl/min, exposure time of 5–7 min. The Ron receptor is a member of a distinct family of multifunctional RTKs that also includes c-Met. Found inside – Page 56On blebs, nicotinic acetylcholine and concanavalin A receptors as well as other molecules ... through the fact that cell shape is largely a function of the ... ConA has also been extensively reported to enhance tumor-cell-based vaccination strategies. For experiments defining the independent binding of Gα or Gβγ described below, surfaces modified with 7000–12,000 RU of ConA have been utilized. lipoproteins and endotoxins) has been confirmed using. William C. Boyd alone and then together with Elizabeth Shapleigh introduced the term "lectin" in 1954 from the Latin word lego-, "chosen" (from the verb legere, to choose or pick out).. However, already in the ‘70s it was demonstrated that ConA can expand tumor reactive T cells in an ex vivo setting. In 2004, a more powerful enrichment method was designed as multi-lectin affinity column (MLAC), which contains several different lectins in the same column (Yang & Hancock, 2004). (A) Treatment of tumor-bearing mice with ConA induces the expansion and activation of CD8+ CTLs and NK cells, which inhibit the formation of hepatic tumor nodules. Europe PMC is an archive of life sciences journal literature. Of note, since ConA-induced T cell activation is not antigen-restricted, systemic injection with ConA has the potential risk to also activate non-tumor reactive T cells, that may lead to immune-related toxicity like autoimmune responses. Concanavalin A (Con A) _______ is a plant lectin protein found in jack-bean that binds to mannose and glucose residues of glycoproteins. 1975 May;72(5):1917-21. doi: 10.1073/pnas.72.5.1917. Interestingly, ConA has been reported to activate NFAT (nuclear factor of activated T cells), a family of transcription factors that are important in the development and function of the immune system, including T cell receptor (TCR) engagement [5]. Found inside – Page 156gated as a function of cell culture density, since it has been demonstrated that cell-contact in culture can lead to surface modifications (Hakomori, 1970; ... transcription factors that are important in the development and function of the immune system, including T cell receptor (TCR) engagement5. Structures of core trimannoside 1, deoxy analogues 2–12, Man 5 oligomannose carbohydrate 13, and biantennary complex carbohydrate 14. T cell activation by concanavalin A in the presence of cyclosporin A: immunosuppressor withdrawal induces, Solubility: 10 mg/ml in water or phosphate buffered saline (PBS), 2018 Virus Res. We use cookies to help provide and enhance our service and tailor content and ads. Found inside – Page 375( The data were calculated with puromycin in the absence of concanavalin A ... of migration ( % ) increases as a function of the amount of concanavalin A. Acute liver failure (ALF) is a serious threat to the life of people all over the world. Unfortunately, at present there is no cure. The dimeric derivative, succinyl-Con A, is just as effective a mitogen as the native lectin, but it lacks the ability to modulate cell surface receptor mobility. Figure 14. Con A-induced liver injury is a commonly used murine liver injury model considered suitable for studying the pathological mechanisms of human viral hepatitis and autoimmune liver disease. sunil yadav. The absence of bacterial contamination (e.g. Subgroups of 5 animals were … The propeptide is split into an N-terminal and a C-terminal part, the gamma and beta chain, respectively. Journal of Biomolecular Structure and Dynamics 1983 , 1 (4) , 961-997. Role of mitochondrial membrane potential in concanavalin A-induced apoptosis in human fibroblasts. DNA and RNA function as genetic material. Major drawbacks of this model include the lack of circulating autoantibodies and rapid hepatocyte damage following a single-dose Con A injection, which is not a typical feature of chronic AIH (Liu et al., 2013). Multi-angle light scattering data highlight the onset of the condensation ... Concanavalin A, amyloid aggregation, ThT binding, conformational changes, light scattering, aggregates structure, AFM. This results in activation, proliferation and differentiation of both CD4 and CD8 T cells. Distinct development and functions of resident and recruited liver Kupffer cells/macrophages. Concanavalin A (Con A)-induced Lymphocyte Proliferation - Measurement of human lymphocyte proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Importantly, these receptors represent pharmacological targets to treat a number of neurological and neurodegenerative disorders. Cell-surface binding specificity of Concanavalin A The lectin Concanavalin A, isolated from the Jack-bean plant Canavalia ensiformis, is an -mannose/ -glucose-binding lectin. Found inside... increases suppressor function, decreases IgE synthesis.9 Concanavalin A, ... observations of Delafuente et al. that concanavalin A induced suppression ... l-Phytohemagglytinin (l-PHA) usually targets the structure of β-1,6-GlcNAc moiety of N-glycans (Abbott et al., 2008; Ahn et al., 2009).
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